RBS

Part:BBa_K4229004

Designed by: Nikita Edel   Group: iGEM22_Freiburg   (2022-09-28)


RBS pCDF-Duet-1 MCS1 and MCS2


This ribosomal binding site (RBS) from pCDF-Duet-1 backbone was used for both multiple cloning sites (MCS1 and MCS2) to initiate the translation of our pathway genes, which are the following Biobricks:

BBa_K42290013, BBa_K42290015, BBa_K42290016, BBa_K42290018, BBa_K42290033, BBa_K42290034, BBa_K42290052, BBa_K42290053, BBa_K42290054 and BBa_K42290055

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None